Effect of Aloe Vera, Wood Ash, and Indole-Butyric Acid on In-Vivo Macro Propagation of Robusta Coffee Stem Cuttings
DOI:
https://doi.org/10.47604/ija.2840Keywords:
Aloe Vera, Coffee Canephora, Indole-Butyric Acid, Cuttings, Wood AshAbstract
Purpose: This study investigated the influence of Aloe Vera, Wood ash and a synthetic growth hormone Indole-butyric acid (IBA) on root and shoot in the proliferation of Coffee Wilt disease-resistant (CWD-R) Robusta coffee stem cuttings.
Methodology: Stem cuttings of CWD-R Robusta coffee were treated with five levels of rooting natural substances and IBA independently and in combination. The treated cuttings were propagated in a uniform potting medium under humidity chamber conditions. Data was collected on the number of surviving cuttings, shoot and root number, and root and shoot lengths. At 90 days after planting, data on root proliferation, the number, and lengths of roots were collected and analyzed for variance (ANOVA) using GenStat 11th Edition.
Findings: The survival, root, and shoot development were significantly (p<0.05) influenced by the rooting substances applied. A combination of Aloe Vera and IBA, 0.8% IBA, and Aloe Vera gel applied alone gave the highest percentages (90, 70 and 70%, respectively) of stem cuttings that survived to form roots and shoots. A mixture of aloe Vera and IBA, 0.8% IBA, and Aloe Vera gave the highest mean number of shoots and roots per cutting; that is (8.75, 8.5, and 8.5 cm) and (13.87, 12.85 and 12.39 cm) for shoots and roots respectively. Cuttings treated with wood ash and control gave the lowest mean number of shoots and roots that formed per cutting; that is (5.25 and 4.50 cm) and (11.75 and 11.38 cm) for shoots and roots respectively.
Unique Contribution to Theory, Practice and Policy: Propagation of Coffea canephora, by stem cuttings can be adequately achieved by using Aloe Vera, IBA, or their mixture, but not with wood ash.
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References
Adham A. R., Zolman B. K., Millius A., Bartel B. (2005). Mutations in Arabidopsis Acyl-CoA oxidase genes reveal distinct and overlapping roles in beta-oxidation. Plant Physiology Journal. 41, 859–874. DOI: 10.1111/j.1365-313X.2005.02343.x
Alvarez, R., S.J. Nissen, E.G. Sutter, (1999): Relationship between Indole-3-Acetic Acid Levels in Apple (Malus pumila Mill) root-stocRN cultured in vitro and adventitious root formation in the presence of indole-3-Butyric Acid. Plant Physiol.89:439-44
AMA (Agribusiness Management Associates, Ltd.) (2015). Final Report on the Evaluation of the Economic Viability and Sustainability of Investing in Production of Coffee Planting Materials, February, prepared for AMA: Feed the Future (FtF) Commodity Production and Marketing Activity.
Andrade J, S (2013). Comparison between grafting and cutting as vegetative propagation methods for conilon coffee plants. Acta Scientiarum. Agronomy, v. 35, n. 4, p. 461-469, 2013.
Bajaj Y. P. S. (1995). Somatic Embryogenesis and Its Applications for Crop Improvement. Somatic Embryogenesis and Synthetic Seed I, 105–125. DOI: 10.1007/978-3-662-03091-2_8.
Bozhkov, P. V., Filonova, L. H., Suarez, M. F. (2005). Programmed cell death in plant embryogenesis. Curriculum of Top Development and Biology, 67: 135–179.
Bryant, G. (2005). Propagation Handbook. Stackpole Books: Mechanicsburg, Pennsylvania.
Chithra, P., Sajithlal, G., Chandrakasan, G. (1998). Influence of aloe vera on the glycosaminoglycans in the matrix of healing dermal wounds in rats. Journal of Ethnopharmacology, 59: 179–86.
Davis R.H.; Aloe vera: A scientific approach. New York: Vantage Press; [Google Scholar]
Dick, J and Aminah, H., (1994). Vegetative propagation of tree species indigenous to Malaysia. Common. For. Rev. 73. 164-171.
Diiro E, (2020); Enhancing Innovation in the Ugandan Agri-Food Sector: Robusta Coffee Planting Material & Tropical Fruit Processing, January 2018SSRN Electronic Journal: DOI: 10.2139/ssrn.4430082
FAOSTAT, (Country profiles: Uganda) December 2013; Rome, Italy
Hakiza, G.J.; Serani, S. and Olal, S., 2021: Preliminary identification of fusarium species from robusta coffee. Unpublished Progress Report on Coffee Wilt Research. NACORI, Kituza, Mukono.
Hartmann H.T, Kester DE, Davies FT, Geneve JRL (1997). Plant Propagation: Principles and Practices, Sixth Edition, Prentice Hall, New Jersey.
Hartmann H.T, Kester DE, Davies FT, Geneve JRL (2002). Plant Propagation: Principles and Practices. Seventh Edition. Prentice Hall. Pearson Education, Inc., Upper Saddle. p. 915.
Hartmann, H. T; D.E. Kester; F.T. Davis, Jr and R.L. 2005; GENEVE. Plant propagation: principles and practices. New Jersey: Prentice Hall.
Healy, M, Mitchell. L., 2008. Hormonal controls of root development in coffee shoot cuttings: role of auxin and NAA. J. Exp. Bot., 59 (9):2361-2370.
Heuser, L., Charles W. (Editor). 2007, Richard B., Mike H., Innes C, Arbury J.,(Contributing Authors), (2007) ; The Complete Book of Plant Propagation, Taunton Press.
Hill K., (2006).; The Centre for Plant Integrative Biology (CPIB); School of Biosciences, University of Nottingham; Loughborough, UK
Holzapfel, P. L. Wessels, B. E. Van Wyk, W. Marais, and M. Portwig, “Chromone and aloin derivatives from Aloe broomii, A. Africana and A. speciosa,” Phytochemistry, vol. 45, no. 1, pp. 97–102, 2001997.View at: 7:00PM 4/3/2022.
International Monetary Fund (IMF), 2022: Financial Outlook for Uganda 2021, Entebbe, Uganda.
Mahipal and Machokali, M. (2013); Impact of Auxins on Vegetative Propagation through Stem Cuttings of Couroupita guianensis Aubl.: A Conservation Approach
Marshall JM. Aloe vera gel: What is the evidence? Pharma Jr. 1990; 24:360–2. [Google Scholar]
Ministry of Agriculture, Animal Industry, and Fisheries (MAAIF). 2016. Agriculture Sector Strategic Plan 2015/16-2019/20. Kampala, Uganda: Uganda: MAAIF. http://npa.ug/wpcontent/uploads/2016/08/ASSP-Final-Draft.pd
Newton AC, Mesen JF, Dick JMcP, Leakey RRB (1992). Low technology propagation of tropical trees: Rooting physiology and practical implications. In: Mass production technology for genetically improved fast growing forest tree species. AFOCEL, Nangis, France 2: 417-424.
Ofori DA, Newton AC, Leakey RRB, Grace J (1996). Vegetative propagation of Milicia excelsa by leafy stem cuttings: Effects of auxin concentraton, leaf area and rooting medium. For. Ecol. Manage., 84(1-3): 39-48.
Pistelli, M.H, and Laxmipriya, P.M; “Evaluation of biological properties and clinical effectiveness of Aloe vera: a systematic review,” Journal of Traditional and Complementary Medicine, vol. 5, no. 1, pp. 21–26, 2015.
Pua, E., Chong, C. & Rousselle, G.L. 1983 In vitro propagation of Ottawa 3 apple rootstock Can. J. Plant Sci. 63 183 188
Rutherford, M. A. 2006. ; Current knowledge of coffee wilt disease, a major constraint to coffee production in Africa. Phytopathology 96:663-666.
Swaibu, M., T. Odokonyero and E. Munyambonera (2014). “Robusta Coffee Nursery Seedling Multiplication Program in mid-Northern Uganda: Opportunities and Challenges” Economic Policy Research Centre (EPRC) Policy Brief, Issue 44 (May).
Toogood, Alan, (1999), American Horticulture Society Plant Propagation: The Fully Illustrated Plant-by-Plant Manual of Practical Techniques, AHS.
Tyler V.; The honest herbal: A sensible guide to the use of herbs and related remedies. 3rd ed. Binghamton, New York: Pharmaceutical Products Press; 1993. [Google Scholar]
UCDA (2022) Coffee Production yearly report, 2012/2022; Uganda Coffee Development Authority, Ministry of Agriculture, Animal Industry and Fisheries.
UCDA (2015) Uganda National Coffee Strategy 2040: Plan for 2015/16 – 2019/20, Uganda Coffee Development Authority, MAAIF.
UCDA (2016) Coffee Production Handbooks (under revision), Uganda Coffee Development Authority, Ministry of Agriculture, Animal Industry and Fisheries.
Wareing, P; (1958). Interaction between indole-acetic acid and gibberellic acid in cambial activity. Nature. 1958;181:1744–1745. doi: 10.1038/1811744a0. [PubMed] [CrossRef] [Google Scholar]
Wu X., Ding. W., Zhong, J. Wan, and Z. Xie (2013). Simultaneous qualitative and quantitative determination of phenolic compounds in Aloe barbadensis Mill by liquid chromatography-mass spectrometry-ion trap-time-of-flight and high performance liquid chromatography-diode array detector. Journal of Pharmaceutical and Biomedical Analysis, 80: 94–106.
Zhang, Y., Zhichao, B. and Xiaoyan, Y. (2018). Chemical Investigation of Major Constituents in Aloe vera Leaves and Several Commercial Aloe Juice Powders. Herbalife Nutrition, Worldwide Research Development and Scientific Affairs, 950 West 190th St.
Ziv, M. (2005). Simple bioreactors for mass propagation of plants, in liquid culture systems for in vitro plant propagation. In: Hvoslef-Eide, A. K. and Preil, W. (Eds). Springer Bioscinces,. Dordrecht Springer, 79–93. DOI: 10.1007/1-4020-3200-5_5. https://link.springer.com/chapter/10.1007/1-4020-3200-5_5
Zolman B. K., Nyberg M., Bartel, B. (2007). IBR3, a novel peroxisomal Acyl-CoA dehydrogenase-like protein required for indole-3-butyric acid response. Plant Molecular Biology. 64, 59–72. DOI: 10.1007/s11103-007-9134-2.
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