EFFECTS OF LONG-TERM ADMINISTRATION OF COOKED BEANS (Vigna unguiculata) DIET ON LEARNING AND MEMORY IN MICE
DOI:
https://doi.org/10.47604/ijf.168Keywords:
Serotonin, 5-Hydroxytryptophan, Learning & Memory, Beans, Vigna unguiculata, MiceAbstract
Purpose: The neurotransmitter, serotonin has neurobehavioural actions which include mood, memory, learning and sleep. Beans, the stable diet of Nigerians contains serotonin and its precursor, 5-Hydroxytryptophan in significant amounts. It was therefore the aim of this study to find out whether long term consumption of cooked beans (Vigna unguiculata) diet has effects on some neurobehavioural parameters notably; learning and memory, using Swiss white mice as experimental animals.
Method: Thirty (30) CD1 mice were randomly assigned into three groups, viz; (10, control, 20 Test) weighing 15-30g were fed normal rodent chow, 50% cooked beans diet (w/w) respectively and serotonin precursor (5-HTP) diet (0.2mg/50g w/w) for thirty days. All the mice had access to clean drinking water ad libitum. Before the neurobehavioural parameters were assessed, the phytochemical analysis of the beans, LD50 of the beans (Vigna unguiculata) and that of the serotonin precursor (5-HTP) were determined. Serotonin concentration was measured in the beans using gas chromatography analysis. Learning and memory, was investigated alongside food and water intake and body weight change. Involvement of serotonin pathway was investigated using the set of mice administered serotonin precursor for comparison with the beans diet fed mice. Mice were tested in the Morris water maze for 8 days: 3 days of acquisition training, 3 days of reversal training, 1 day of single probe trial and 1 day for visible platform task.
Results: Learning on days 1, 2 & 3 of acquisition training was improved in the cooked beans and serotonin precursor diet-fed mice when compared to control (p<0.001, respectively). The trend was similar during the reversal training, memory was improved in the beans and serotonin precursor diet-fed mice when compared to control (p<0.001). During the probe trial, the swim duration in the South-East quadrant was significantly higher for cooked beans and serotonin precursor group compared to control (p<0.05). However, during the visible platform task, the swim latencies for the cooked beans and serotonin precursor group was significantly lower compared to control. Our finding therefore suggests that long term consumption of beans diet enhances learning and memory.
Conclusion and Recommendation: In conclusion, long term consumption of cooked beans diet enhances learning and memory. If this research can be extrapolated to humans, we recommend that the serotonergic potential of beans may be harnessed in the prevention, treatment and management of behaviour/ mental disorders as this could potentially open up a vista for improved mental health status in populations globally.Downloads
References
Adeyele,E.J.(1995).Studies of Chemical Composition and functional properties of African Yambeans(Spensotylis Stenoorpa) flour.Ph,D Thesis,Department of chemistry,Federal University of Technology,Akure,Ondo State,Nigeria.
Akirav, I., Sandi, C. and Richter-Levin, G (2001).Differential activation of hippocampus and amygdala following spatial learning under stress. European Journal of Neuroscience, 14, 719-725.
Benice, T.S., Rizk, A., Kohama, S., Pfankuch, T.and Raber, J (2006). Sex-differences in age-related cognitive decline in C57BL/6J mice associated with increased brain microtubule-associated protein 2 and synaptophysin immunoreactivity. Neuroscience 137:413-423.
Bennett, J.C., McRae, R.A., Levy, L.J. and Frick, K.M (2006). Long term continuous, but not daily environmental enrichment reduces spatial memory decline in aged mice. Neurobiology of learning and memory,85,139-152.
Bennick, E., Maurice, O. and Elizabeth R. (2008).Beans & Health: A comprehensive Review, Frazee, MN.
Brunton, L.B., Lazio, J.S. and Parker, K.L (2005).Therapeutics. The Pharmacological Basis of Therapeutics (607-629).New York: McGraw-Hill.
Cacciopo, J.T and Gardener, W.L (1999).Emotion. Annual Review of Psychology, 50(1), 191-214.
Chen, G., Chen, K.S., Knox, J., Inglis, J., Bernard, A., Martin, S.J, Justice, A., McConlogue, L., Games, D., Freedman, S.B. and Morris, R (2000). A learning deficit related to age and beta-amyloid plaques in a mouse model of Alzheimer's disease. Nature, 408, 975-979
Daniel, L.C. and Michael, R.K. (2007).Biogenic amine neurotransmitters in C.elegans.Wormbook(pp.1-15).Panadesa: Worm Book.
Davies, K.G., Edagha, N., Aribo, E., Antai, A.B. and Osim, E.E (2013).Effects of artemether and Artesunate on social behaviour and pain perception. Research in Neuroscience, 2(3), 31-38.
Ganong, W.F., Barrette, K.E., Berman, S.M., Boitano, S. and Brooks, H.l (2010).Ganong's Review on Medical Physiology (pp.76-230).New Delhi: McGraw-Hill.
Gatel, F. (1994).Quality of legume seeds for Non-Ruminant Animals. Animal Feed Science and Technology, 45(3):317-348.
Gref, E. and Eaton, J.W (1993).Suppression of Caloric Cancer by Dietary Phytic Acid. International Journal of Nutrition and Cancer, 19(1):11-19.
Kovalev, G.V., Sahin, V.A. and Lanhskaia, A.V (1989).Psychotropic effects of glutamic acid diethyl ester in mice.Biull EKSP Biol.Med; 108(9):302-4.
Lelei, S.A., Osim, E.E., Nneli, R.O. and Bisong, S.A (2012).Chloroquine phosphate administration improves learning and memory in mice. European Journal of Scientific Research, 93(3), 372-377.
Messman, T. (2005).Psychiatric drugs: Chemical warfare on humans: Interview with Robert Whitaker. Retrieved August 27, 2005 from http:/www.naturalnew.com.
Mcdonald, R.J. and White, N.M (1994).Parallel information processing in water maze: Evidence for independent memory systems involving dorsal striatum and hippocampus. Behaviour Neural Biology, 61,260-270.
Morris, R (1984).Developments of a water-maze procedure for studying spatial learning in the rat. Journal of NeuroscienceMethods, 11, 47-60.
Osim, E.E (2012).Our consumables and our emotions. Faculty of Basic Medical Science, University of Calaber, lecture series, july11, 2012.
Portas, C.M., Bjorvatn, B. and Ursin, R (2000).Progress in Neurobiology, 60(1), 13-35.
Shansudden, A.M. and Elsayed A (1998).Suppression of large intestinal Cancer in F344 rats by Inositol Hexaphosphate.Carcinogenesis, 9(4):577-580.
VanderPoel, A.F.B., Mollee, P.W., Huisman, J.and Liner, I.E (1990b).Variationsamong species of animals in response to the feeding of heat-processed beans.Bean processing and effects on growth, digestibility and organ weights in piglets. Livestock Production Science, 25(i-2):121-135.
Walther, D.J., Peter, J.U., Winter, S., Holtz, M., Paulmann, N., Grohmann, M., Vowinctel, J., Alamo, B.V., Wilhem, C.S., Ahnert, H.G. and Bader, M (2003).Serotonylation of GTPases is a signal transduction pathway that triggers platelet alpha-granules release.Cell.115(7),851-862.
Wong, A.A. and Brown, R.E (2006). Age-related changes in visual acuity, learning and memory in C57BL/6J and DBA/2J mice. Neurobiology of Ageing, online28-Sept-2006. Doi:10.1016/j.neurobiolaging.2006.07.023.
Wortman,C.S (2006).Phaseolus vulgaris(Common beans).Record from PROTAAU.Brink, M. and Basey, G(Editors).PROTA(Plant Resources of Tropical Africa) Netherlands.
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