SERUM AMH, INHIBIN B, FSH AND ESTRADIOL PROFILE IN WOMEN SEEKING CONCEPTION THROUGH IN VITRO FERTILIZATION IN NIGERIA
Keywords:
Ovarian Reserve, Anti-Mullerian Hormone, Follicle Stimulating Hormone, Inhibin B, Estradiol.Abstract
Purpose: This study was designed to evaluate the predictive ability of AMH, inhibin B, Follicle stimulating hormone and estradiol as biomarkers of ovarian reserve in assisted reproductive technology.
Methodology: Thirty (30) female partners of infertile couples seeking assisted reproductive technology through in vitro fertilization (IVF) and twenty seven (27) fertile women who are oocyte donors were recruited from Life Life Hospital, Lily Hospitals and Shepherd Fertility Hospital in Southern Nigeria from April to November 2017 and evaluated for AMH, inhibin B, estradiol and FSH using Enzyme-linked Immunosorbent assay technique. Subjects were divided into four groups (A,B,C,D) on the basis of age. A: Oocyte donors < 23 years; B: Oocyte donors ≥ 23years; C: Female partners of infertile couples <35years; D: Female partners of infertile couples ≥35years. Blood sample was collected from each participant on the day 3 of their menstrual cycle.
Findings: Mean AMH serum levels for female partners of infertile couples and oocyte donor were 4.26±1.7ng/ml and 5.13±1.12ng/ml respectively (p=0.04); mean FSH serum levels for female partners of infertile couples and oocyte donor were 8.64 ±3.40mIU/ml and 6.37±1.48mIU/ml, respectively (p=0.02); mean inhibin B serum levels for female partners of infertile couples and oocyte donors were 60.57±13.99 pg/ml and 77.52±12.33pg/ml respectively (p=0.00); mean estradiol serum levels for female partners of infertile couples and oocyte donors were 69.38±18.83pg/ml and 44.06±8.70pg/ml respectively (p=0.00).
Unique contribution to theory, practice and policy: AMH has a stronger predictive power for ovarian reserve function than FSH only in women who are 35 years and above.
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