SERUM AMH, INHIBIN B, FSH AND ESTRADIOL PROFILE IN WOMEN SEEKING CONCEPTION THROUGH IN VITRO FERTILIZATION IN NIGERIA

Authors

  • Nosakhare O. Osakue Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria
  • Chinedum C. Onyenekwe Department of Medical Laboratory Science, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria
  • Joseph E. Ahaneku Department of Medical Laboratory Science, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria
  • Gabriel O. Igberase Shepherd Fertility Hospital, Warri, Delta State, Nigeria.
  • Joseph I. Ikechebelu Department of Obstetrics and Gynaecology, Nnamdi Azikiwe University Teaching Hospital
  • Louis O. Alekwe Lily Hospitals LTD, Warri, Delta State, Nigeria.

Keywords:

Ovarian Reserve, Anti-Mullerian Hormone, Follicle Stimulating Hormone, Inhibin B, Estradiol.

Abstract

Purpose: This study was designed to evaluate the predictive ability of AMH, inhibin B, Follicle stimulating hormone and estradiol as biomarkers of ovarian reserve in assisted reproductive technology.

Methodology: Thirty (30) female partners of infertile couples seeking assisted reproductive technology through in vitro fertilization (IVF) and twenty seven (27) fertile women who are oocyte donors were recruited from Life Life Hospital, Lily Hospitals and Shepherd Fertility Hospital in Southern Nigeria from April to November 2017 and evaluated for AMH, inhibin B, estradiol and FSH using Enzyme-linked Immunosorbent assay technique. Subjects were divided into four groups (A,B,C,D) on the basis of age. A: Oocyte donors < 23 years; B: Oocyte donors ≥ 23years; C: Female partners of infertile couples <35years; D: Female partners of infertile couples ≥35years. Blood sample was collected from each participant on the day 3 of their menstrual cycle.

Findings: Mean AMH serum levels for female partners of infertile couples and oocyte donor were 4.26±1.7ng/ml and 5.13±1.12ng/ml respectively (p=0.04); mean FSH serum levels for female partners of infertile couples and oocyte donor were 8.64"‰±3.40mIU/ml and 6.37±1.48mIU/ml, respectively (p=0.02); mean inhibin B serum levels for female partners of infertile couples and oocyte donors were 60.57±13.99 pg/ml and 77.52±12.33pg/ml respectively (p=0.00); mean estradiol serum levels for female partners of infertile couples and oocyte donors were 69.38±18.83pg/ml and 44.06±8.70pg/ml respectively (p=0.00).

Unique contribution to theory, practice and policy: AMH has a stronger predictive power for ovarian reserve function than FSH only in women who are 35 years and above.

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Author Biographies

Nosakhare O. Osakue, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria

Post Graduate Student

Chinedum C. Onyenekwe, Department of Medical Laboratory Science, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria

Lecturer

Joseph E. Ahaneku, Department of Medical Laboratory Science, Nnamdi Azikiwe University, Nnewi Campus, Anambra State, Nigeria

Lecturer

Gabriel O. Igberase, Shepherd Fertility Hospital, Warri, Delta State, Nigeria.

Author

Joseph I. Ikechebelu, Department of Obstetrics and Gynaecology, Nnamdi Azikiwe University Teaching Hospital

Lecturer

Louis O. Alekwe, Lily Hospitals LTD, Warri, Delta State, Nigeria.

Author

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Published

2019-10-11

How to Cite

Osakue, N. O., Onyenekwe, C. C., Ahaneku, J. E., Igberase, G. O., Ikechebelu, J. I., & Alekwe, L. O. (2019). SERUM AMH, INHIBIN B, FSH AND ESTRADIOL PROFILE IN WOMEN SEEKING CONCEPTION THROUGH IN VITRO FERTILIZATION IN NIGERIA. Journal of Health, Medicine and Nursing, 4(5), 23 – 34. Retrieved from https://iprjb.org/journals/index.php/JHMN/article/view/986

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