Potentials of Scent Leaves (O. gratissimum) as Feed Additive on Performance, Selected Blood Biochemicals and Helminthiasis in Weaner Pigs
DOI:
https://doi.org/10.47604/jlp.v2i1.2117Keywords:
Oocysts Shedded, Kepro Dewomer, Scent Leaves, Phytochemical, Supplementation, Weaner PigsAbstract
Purpose: A study was conducted to examine the potentials of scent leaves (SL) (O. gratissimum) meal as a feed additive on performance, blood biochemicals and antihelmintic in weaner pigs.
Methodology: Completely Randomized Design (CRD) was adopted using 30 mixed breed of large-white and duroc weaner pigs, allocated to three treatments with ten pigs each, replicated five times with two pigs per replicate. Formulated diet (1) is a Positive Control (PC) - supplemented with Kepro-dewomer, diet 2 a Negative Control (NC) - without Kepro-dewomer and Scent Leaves (SL), while Diet 3 was supplemented with Scent Leaves (SL) at 1.0%. Data collected were analysed using ANOVA with SAS software. Treatment means were separated using Duncan's New Multiple Range Test.
Findings: Results indicated that there were significant (p<0.05) differences among treatments in final body weight gain (FBWG). Highest daily weight gain (HDWG) (0.51kg) and highest daily feed intake (HDFI) (0.46kg) were recorded in pigs fed diet 3. Packed cell volume (PCV), Red blood cell (RBC), Haemoglobin (Hb) levels were not significantly (p>0.05) different among the experimental groups 1 and 3: (37.01) (26.7), (12.72) (39.11) (25.5) (12.88) respectively. Meanwhile, diets 1 and 3 were significantly (p<0.05) improved serum biochemicals parameters (Aspartate amino transferase - AST). (Alanine aminotransferase - ALT) and (Alkaline phosphate - ALP) compared with diet 2. Kepro dewomer and scent leaves dietary supplementation had a significant reduction on oocysts shedded per gram of feaces in diet 1 (30.42, 31.03, 32.96) and diet 3 (36.78, 35.89, 31.11) compared with diet 2 (61.3, 63.41, 68.01) respectively
Unique Contribution to Theory, Practice and Policy: The idea of alternative medication theory to synthetic drug in livestock feeds was conceived and developed; towards solving the problem of resistance of microbial pathogens to synthetic antibiotic as a result of its continuous usage. Findings, therefore, showed that the strategic supplementation of scent leaves meal as an alternative deworner to synthetic deworner that poses health risks to consumers could be avoided. Also, the need to adapt a collection and processing strategy at many locations wherever the scent leaf plant is produced and are poorly utilized or being wasted.
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References
Abdelheg Barberis, Nadir, Omar, B., Ammar, A and Amir, A (2015). Effect of using an anticoccidial and a prebiotic on production performances, immunity status and coccidiosis in broiler chickens. Asian Journal of poultry science 9:133 - 143
Adedoyin, A. A. (2018) Potentials of Hot Red Pepper (Capsicum annum L.) on performance and coccidiosis in broilers. In: Animal science Association of Nigeria and Nigerian institute of Animal science 7th ASAN - NIAS Joint Annual meeting. Book of proceedings 23rd Annual conference 9th - 13th Sept. 2018 Ilorin, Pp 40 - 44.
Adedoyin, A.A., Mosobalaje, M.A. and Bamimore, A.I. (2019). Performance, Immunostimulating and Blood Biochemical Indices of Broiler Chickens Fed Hot Red Pepper (Capsicum annum L.) Supplemented Diets. Journal of Experimental Agriculture International 36(3):1-8. JEA 1.34966.
Adegbenro, M. Agbede, J.O, Onibi, G.E., and Aletor, V.A (2018). Performance and meat quality of growing pigs fed composite leaf meal premix as an alternative to commercial premix. Inter journal of environment. Agriculture and Biotechnology (IJEAB) Vol 3(1) 66 - 71.
Adegbesan, S.I. and Abdulraheem, I. (2020). Growth Performance nutrient utilization, haematology and serum biochemistry of African Catfish (Clarias gariepinus) broodstock fed varying levels of Aspilia africana leaves-paste. Nigerian Journal of Animal Production 47(1):129-139.
Adejinmi, J.O, Sadiq, N.A, Fashanu, S.O., Lasis, O.T and Ekundayo, S (2004). Study on the blood parasite of sheep in Ibadan, Nigeria. African Journal of Biomedical Research 7, 42 - 43.
Ademola, I.O. and Onyiche, T.E. (2013) Haemoparasites and Haematological parameters of slaughtered Ruminants and Pigs at Bodija .Abattoir, Ibadan, Nigeria. African Journal of Biomedical Research 16: 101- 105.
Adenaike, E. A. (2020). Effect of halminthosis on some blood parameters of pigs in Ibadan, Nigeria. Nigerian Journal of Animal production Vol 47(1): 53 - 57.
Agbede, J.O. and Aletor, V.A. (2003). Evaluation of fish meal replaced with leaf protein concentrate from glyricidia in diets for broiler-chicks: Effect on performance, muscle growth, haematology and serum metabolites. International Journal of Poultry Science 2(4):242 - 250.
Akbarian, G.A., Kermanshashi, H., Gilani, A. and Moradi, S. (2012). Influence of turmeric rhizome and black pepper on blood constituents and performance of broiler chicken. African Journal Biotechnology II: 8606 - 8611.
Alexander, P. (2016) Phytochemical Screening and mineral composition of the leaves of scent leaf (Ocimum gratissimum). International Journal of Applied Sciences and Biotechnology 4(2): 161-165.
Al-Harthi, M.A. (2006). Impact of supplemental feed enzymes, condiments mixture or their combination on broiler performance. Nutrients digestibility and plasma constituents. Internal journal of Poultry Science. 5(8), 764-771.
Allan, R. and Peter, N. (1998). Epidemiology, Diagnosis and control of Helminth parasites of swine. Food and Agriculture Organization of the United Nations, Rome FAO Animal Health Manual 159pp.
Amala, I.A. and Okorodudu, E.O. (2016). Comparative Evaluation of the Growth Performance and Feed Intake of Weaned Rabbits Fed Tropical Grasses and selected forages leaves. Int. Journal of Research Studies in Agricultural Sciences (IJRSAS). 2(2): pp 14-18.
Anyanwu, M. (2010). Evaluation of the feed preservative potentials of Ocimum gratissimum. An unpublished B.Sc. research project, Department of Animal Science, Federal University of Technology, Owerri.
AOAC (2006). Association of Official Analytical chemists. Official methods of Analysis 15th ed. Washington, D.C. Procedure number, 1230.
Arhoghro, E.M., Ekpo, K.E and Ibeh, G.O (2009). Effect of aqueous extract of scent leaf (O. gratissimum) on carbon tetrachloride (CCl4) induced liver damage in albino wister rats. African Journal of Pharmacy and Pharmacology Vol. 3(11): 562 - 567.
Arogbodo, J.O, Osho, I.B Faluyi, O.B and Awoniyi, T.A M. (2020). Haematological indices of Salmonella Gallinarum (Gr. Di - 1, 9, 12) Infected broiler chickens treated with ethanolic leaf extract of chrysophyllum albidum (G. Don). Nigerian Journal of Animal Production 47(1) 65 - 80.
Babatunde, G.M., Fahimi, A.O. and Oyedeji, A.O. (1992). Rubber seed oil versus palm oil in broiler chicken diets: Effects on performance, nutrient digestibility, haematology and carcass characteristics. Anim. Feed Sci. Tech. 25:133-146.
Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods- a review. International Journal of Food Microbiology, 94:223-253.
Cardozo, J. and Fred, M. (2004). Species: A global History. Reaction Books. Pp. 128.
Cardozo, P.W., Calsamiglia, S., Ferre, t A. and Kamel, C. (2004). Effect of natural plant extracts on ruminant protein degradation and fermentation profiles in continuo's culture J. Animal Sci. 82:323-3236.
Chesson, A., steward, C.S. and Wallace, R.J., (1982). Influence of plant phenolic acid on growth and cellulytic activity of rumen bacterial. Applied Environ Microbiol., 44:597-603.
Chrubasik, S.M., Pittler, M. H and Roufogalis B.D. (2005) Zingiberis rhizome: A comprehensive review on the ginger effect and efficacy profiles. Phytomedicine 12:684 - 701.
Cuppett, S.L. and Hall, C.A. (1998). Antioxidant activity of Labiatae. Food and Nutrition Research. 42, 245-271.
Duncan, D.B (1965) Duncan Multiple range Tests Biometrics 11:1.
Fajimi, A.K and Taiwo, A.A. (2005). Herbal remedies in animal parasitic diseases in Nigeria. A review African journal of Biotechnology 4: 303 - 307.
Galani, A., Kermanshashi, H., Golian, A., Tahmasbi, A. and Aami, M. (2018). Appraisal of haematological indices and humoral immunity in commercial laying hens fed rations consisting cotton seed meal and sodium bentonite. Iranian Journal of Applied Anim. Science. Archive of SID.
Ghazalah, A.A and Ali, A.M (2008). Rosemany leaves as dietary supplement for growth in broilers. International Journal of Poultry Science, 7(3): 234 - 239.
Hashemi, S.R., Zulkifl Hair-bejo, M., Karami, M. and Soleimani, A.F. (2009). The effects of Euphorbia hirta and acidifier supplementation on growth performance and anti-oxidant activity in broiler chickens. Proceedings of the 21st Vet. Association Malaysia (VAM) Congress, Aug. 7 - 9. Port Dickson, Malaysia, Pp: 215 -217.
Heiko, N. (2019). Applied biosystems - Guidelines for taking diagnostic sample from pigs faeces. Thermo Fishers Scientific. Royal Veterinarian Society UK 6pp.
Idodo-Umeh G. (2011). College biology. 4th Edition. Idodo-Umeh Publishing. Benin City, Nigeria pg. 657.
Ijeh, I.I., Omodamiro, O.D. and Nwanna, I.J. (2005). Antimicrobial effects of aqueous and ethanolic fractions of two species O. gratissimum and xylopia aethiopica. African Journal of Biotechnology 4:953 -956.
Iwu, M. M. (1993). Hand book of African Medicinal plants. CRC Press. Boca Raton Pp 214 - 215.
Jain, NC (1993) Essential of veterinary Haemetalogy, Lea and farbiger, Philadolphia pp 20 - 35.
Junaid, S., Olabode, A.O., Onwuliri, F.C., Okwori, A.E.J and Agina, S.E. (2006). The antimicrobial properties of Ocimum gratissimum extracts on some selected bacterial gastrointestinal isolates. African Journal of Biotechnology, 5(22), 2315-2321.
Kahn, C.M. (2005). The Merch Veterinary Manual 9th edition. Cynthia M. Kahn and Scott line (Eds). Merck & Co. Inc. Rahway, Us.A 2344pp.
Kamel, C.T. (2001). Modes of action of plant extract in non-ruminant: In Recent advances in Animal Nutrition. P.C. Garasssiorthy and J. Wiseman (eds.), Norhinghan Union press Morthinghan U.K. pp. 387.
Kaneko, J.J. (1989). Clinical biochemistry of domestic animals. 4th Edition. Academic Press. Inc. New York. Pp 102-122.
Lamb, G.N (1991) Manual of veterinary laboratory technique. (CIBA - GEIGY) Kenyi, 92 - 109.
Lambert, RJW, Skandamis PN, Coote PJ and Nychas GJE (2001). A study of the minimum inhibitory concentration nd mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology. 91:453-462.
Levic, J., Sredanovic, S., Duragic, O. Levic, L.J and Pakov, V. (2017). New feed additives based on Phytogenics and acidifiers in animal nutrition Biotech in Anim Husbandry 23 (5 - 6) 527 - 534.
Li, PF, Piao XS, Ru YJ, Han X, Xue LF, Zhang HY. 2012. Effects of adding essential oil to the diet of weaned pigs on performance, nutrient utilization, immune response and intestinal health. Asian-Aust J Anim Sci. 25:1617-1626.
Mercy, G.A, Light, F.W and Gospel, A (2017). Qualitative and Quantitative phytochemical screening of some plants used in ethnomedicine in the Niger Delta Region of Nigeria. Journal of Food and Nutrition Sciences 5(5): 198 - 205.
Mitruka, B.M and Rawnsley, H.M (1977). Clinical biochemical and haematologicalm reference values in normal experimental animals. Mason, New York, 42 - 45.
Mohsen Mohammadi Gheisar and In Ho Kim (2017). Phytobiotics in poultry and swine nutrition - a review. Italian Journal of Animal Science.
Muhammad, J., Fazil-Raziq, D. Abdul H., Rifatullah, K. and Ijaz, A. (2009). Effects of aquous extract of plant mixture on carcass quality of broiler chicks. Animal Research Publishing Network (ARPN) Journal of Agricultural and Biological Sciences, 4(1): 37-40.
Nakatani, N. (2000). Phenolic antioxidant from herbs and spices. Biofactors, 141-146.
National Research Council (2012). Microlivestock: Little-known Small Animals with a promising Economic Future - Washington DC: The National Academics Press. https://doi.org/10.17226/1831.
Naubol, D.L. Boniface, N.B., Helen, D.N Charity, A.A Deborah, M.A., Peterside, R.K and Mary M (2016) Establishment of reference values for some biochemical and haematological .parameters for broilers and Layers in plateau State. Nigerian. Vom Journal of veterinary science vol 11: 30 - 35.
Ngozi, D.U., Ohalete, C.N, Ibiam, U.K and Okechuckwu, R.I (2015), Medicinal plants effectiveness against helminthes of cattle Journal of Applied Biosceiences 86: 7900 - 7917 ISSN 1997 - 5902.http://dx.doi.org/10.4314/jab.v86il.6.
Nwachukwu, E. (2009). Evaluation of the antioxidative properties of Monodora nynsrica (African nutmeg), B.Sc project. Micheal Okpara University of Agriculture, Umudike.
Nwakpu, P.E., Uchewa, E.N. and Nweke, F.N. (2010) Growth performance and haematological traits of weaner pigs fed graded levels of Raw Bambara Nut (Vigna Subterranean (L) Verdc) waste. Nigerian Journal of Biotechnology Vol 21 (65 - 70).
Oboh, G. (2004). Antioxidant and antimicrobial property of Ocimum gratissimum leaves. Book of abstracts: 24th Annual Conference of Nigerian society of Biochemistry and Molecular Biology Pp66.
Odoemelam, V.U., Etuk, I.F., Ndelekwute, E.K., Iwuji, T.C. and Ekwe, C.C. (2013). Herbs and Spices: Options for Sustainable Animal Production. Journal of Biology, Agriculture and Healthcare. 3(7): 116-123.
Odoemelam, V.U., Nwaogu, K.O., Ukachukwu, S.N., Esonu, B.O., Okoli, I.C., Etuk, E.B., Ndelekwute, E.K., Etuk, I.F., Ogbuewu, I.P. and Kadurumba, O.E. (2012). Performance of Broiler Chuckens Fed Ocimumgratissimum L. Supplemented diets. Proceedings of 6th Annual Conference of Nigeria Soceity for Indigenous Knowledge and Development (NSIKAD), June 5th - 7th MOUA, Umudike,Pp. 15-16.
Ofokansi, K., Adikwu, M. and Esimore, C.O. (2003). Antibacterial activity of the leaf extract of Ocimum gratissimum (Fam. Labiatae) Bio-Research Vol. 1(1) 35-42.
Oladunmoye, M.K (2006) Immunostimulatory Activity ethanolic leaf extract from ocimum gratissimum in albino rat orogastrically dosed with Escherichia coli (NCIB 86). Journal pharrmacol. Toxicol 1: 389 - 94.
Olomu, J.M (2011). Monogastic Animal Nutrition-Principle and Practices. 2ndedu. St Jackson Publishing Benin City Nigeria 478Pp.
Osuji, P., Fernandez-Riviera, O.S. and Odenyo, A. (1995). Improving fibre utilization and protein supply: Inm animal Fed poor quality Roughages ILRI nutrition Research and plans. Wallace, R.J. and Lahlon-Kassi, A. (ed) Rumen Ecology Research and planning Vol. 1 International Livestock Research Institution Addis-Ababa Pp: 1-22.
Plail, R. 2006. "˜The Innovative Power of Probiotics', Poultry International. Pp. 34-36
Plate1, K. and Srinivasan, K. (2004). Digestive Stimulant action of spices: A myth or reality? Indian J. Medical Research 119:167 - 179.
Prabhu, K.S., Lobo, R., Shirwaikar, A.A. and Shirwaikan, A. (2009) Ocimum gratissimum: A review of its chemical pharmacological and ethno medicinal properties. The open Complementary Medicine Journal, 1:1 - 15
Pradeep C.R. and Kuttan G. (2004). Piperine is a potent inhibitor of nuclear factor-kB (NF-kB), c-Fos, CREB, ATF-2 and Pro-inflammatory cytokine gene expression in BT6F-10 melanoma cells. International Immuno-pharmacology, 2004;4:1795-1803.
Ross, J.G., Christie, G., Holliday, W.G. and Jones, R.M. (1978). Haematological and blood chemistry comparison values for clinical pathology. Vet. Record. 102:29-31.
SAS Institute Incorporation (2012) SAS/STAT User's Guide. SAS Institute Incorporated cary, NC.
Talabi, A.O., Oyekunle, M.A., Abiola, J.O., Makinde, G.E.O, Akinleye, S.O., Ettu, R.O. and Oyejobi, Y.A. (2010). Prevalence of swine diseases in Ijebu division of Ogun State. Nigerian Journal of Animal Production 38:125 - 129.
Talabi, A.O., Oyekunle, M.A., Onasanya, A.S., Tijani, L.A., Sosanya, O.S. and Ettu, R.O. (2004). Comparison of the efficacies of Diazinon + Albendazole, lvomec and lvojec on the control of gastrointestinal nematodes and ectoparaistes of pigs. African Journal of livestock Extension 3:55 - 58.
Tewe, O. O. (2004). Cassava for livestock feed in sub-Sahara Africa. IFAD and FAO Publications. 69pp
Tian, W. (2008). Herbs in Africa. A field guide to the natural history of herbs NSW department of primary industries http//www.ozelacademy.com/8)ojas%2520786%2520.
Tipu, M.A., Pasham T.N. and Ali, Z. (2002). Comparative efficacy of salinomycin sodium and Neem fruit (Azadirachta indica) as feed additives anticocidials in broilers. Int. J. poult. Sci, 1(4); 91-93.
Ukorebi, B.A., Akpet, S.O and Gbose, P.N (2019) Heametology, serum biochemistry and organ histopatology of broiler chickens fed graded dietary levels of Gongronema latifolia (Utasi) Nigerian Journal of animal production 46(2) 164 - 175
Usip, L.P.E (2014) Blood Parasites of Slaughtered Pigs in Uyo Local Government Area, Akwa Ibom State Nigerian Journal of Agriculture, Food and Environment 10:108 - 112.
Windisch, W., Schedle, K., Plitzner, C. and Kroismayr, A. (2008). Use of phytogenic products as feed additives for swine and poultry, Journal of Animal Science, 86:140-148.
Zhou, H., Chen S, Wang, M., and Cheng, A. (2014). Interferons and their receptors in birds: A comparison of gene structure, phylogenetic analysis, and cross modulation. International Journal of Molecular Science. 2014;15:21045-21068.
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