EFFECTS OF PHOSPHORUS AVAILABILITY ON NODULATION AND NITROGEN UPTAKE BY THREE LEGUME CROPS IN TWO GHANAIAN SOILS
DOI:
https://doi.org/10.47604/ija.1309Keywords:
Rhizobia, Rhizobium, Nzema, Adenta, Nodules, Aluminum, desorpAbstract
Purpose: A greenhouse study of Soybean, Cowpea and Pigeon Pea was made at University of Ghana, to determine the effects of phosphorus availability on nodulation and nitrogen uptake by the afore mentioned legumes in two Ghanaian soils, Adenta and Nzema series.
Methodology: Three P rates of 0mg, 50mg and 100mg P of TSP and TPR were applied to a kilogram of soil per pot in two soil series. The pots were arranged using Randomized Complete Block Design. GenstatR was used to do the statistical analysis.
Findings: The results from this study showed that with or without Triple Super Phosphate (TSP) or Togo Phosphate Rock (TPR), soybean did not form nodules in the Nzema soil but nodulated with TSP application in the Adenta soil. The absence of nodulation even with high P from TSP by soybean in Nzema soil is surprising but the observation in the Nzema soil may be attributed to the absence of soybean Rhizobium cells or insignificant numbers of these rhizobia. The results of the present data indicated that treatments with higher P application gave higher N uptake and showed the link among P application, high nodule dry weight, and N uptake. The improvement in the dry matter yield of pigeon pea and cowpea on Adenta soil and soybean on the Nzema soil at TPR50 and TPR100 show the importance of P application to dry matter yield of legumes. Significant difference that was shown by cowpea on Nzema soil could be attributed to the ability of the crop to desorp P from sparingly available P sources through exudation of high amounts of organic acid anions, mainly citrate.
Unique contributions to theory, practice and policy: In the soil, Rhizobium species must recognize their specific host before nodulation may take place and the absence of the appropriate Rhizobium species with the introduction of a legume into a given soil may result in no or poor nodulation. Low P availability is a challenge for crop species to nodulate since the rhizobia responsible for nitrogen fixation have a high P requirement. The toxicity of Aluminum to rhizobia may be due to inhibition of DNA replication because of binding of Aluminum to DNA. One factor that could have accounted for the better nodulation in soil is the higher acidity. The optimum pH for effective rhizobia growth in soils is between pH 6 and 7.
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