Determination of the Chemical Properties of Honey from Suba Region, Homa Bay County-Kenya

Authors

  • Linet Kwamboka Ondogo Kenyatta University
  • Dr. Eng. Daniel Okanga Oyoo Kenyatta University
  • Dr. Robert Motari Bichanga Kenyatta University

DOI:

https://doi.org/10.47604/ijf.2240

Keywords:

Honey, Chemical Properties, Codex Alimentarius Commission

Abstract

Purpose: The purpose of this study was to determine the chemical properties of honey produced in the Suba region, specifically in the Suba South Sub-County, Gwassi North and South divisions' four regions, Nyandiwa (Western), Tonga (Northern), Magunga (Eastern), and Nyancha (Central) of Homa Bay County, Kenya. The study focuses on assessing the chemical properties of honey such as pH, free acidity, HMF content, proline content, glucose content, fructose content, sucrose content and mineral concentrations (K,Na,Ca,Mg,Fe,Zn,and Mn) to ascertain whether they are within the acceptable limits  as set by the Codex Alimentarius Commissions.

Methodology: An experimental approach was used to investigate the various chemical properties of honey sourced from the four regions of the Suba region in Homa Bay County. Codex Alimentarius Commission's defined methods were used to analyze each property. Both primary and secondary data were collected using interviews and purposive sampling techniques to collect a total of 40 honey samples.  A pH meter, UV-visible spectrophotometer, and HPLC instruments were used to measure the pH, free acidity, HMF content, proline content, fructose content, glucose content, and sucrose content of the honey, respectively. The levels of minerals were measured using AAS for Na and K, while Ca, Zn, Mg, Mn, and Fe using a flame photometer. Statistical Package for Social Sciences version 21 was used to perform various statistical tests and generate graphical representations of data. The collected samples were analysed at the Kenyatta University Research Laboratory.

Findings: The findings showed that the chemical properties had the following mean values for the honey sample: pH = 4.01± 0.03, free acidity = 35.83±0.45 mEq/kg, HMF = 19.95±1.76 mg/kg, proline = 629.77±9.80 mg/kg, sum of glucose and fructose = 68.37±0.51%. While for the minerals, manganese was the least common and potassium the most common. The mean values result of this study show that honey from the Suba region satisfies the set standards by Codex Alimentarius Commission.

Unique Contribution to Theory, Practice and Policy: The study validates the theory of international standards in honey production, emphasizing the positive impact of aligning with global benchmarks on honey quality and market acceptance in the Suba region. It emphasizes the importance of quality certification, which contributes to consumer trust and economic growth. The research also supports the theory through sensitization and education, which increase awareness about honey quality and educate beekeepers on proper production techniques. The study advocates for economic diversification through sustainable apiculture, resulting in improvements in economic stability and financial well-being in the Suba region.

Downloads

Download data is not yet available.

References

Adebiyi, F.M., Apan I., Obiajuwa, E. and Olaniyi, H.B. (2004). Chemical and physical characterization of Nigerian Honey. Pakistan Journal of Nutrition, 56 (4): 1034.

Ahmed, K and Karaman S. (2007). Rheological and some physiochemical characteristics of selected Turkish honeys. Journal of Texture Studies, 39: 17 - 27.

Al-Farsi, M., Al-Belushi, S., Al-Amri, A., Al-Hadhrami, A., Al-Rusheidi, M., & Al-Alawi, A. (2018). Quality evaluation of Omani honey. Food chemistry, 262, 162-167.

AOAC (1990). Official methods of Analysis. In Helrich K. 15th edition. Arlington, VA, USA: Association of official Analytical Chemists.

Asif, K., Saeeda, R., Nouman, R., Tabassum, H., and Rawalpiddi, L. (2002). Comparative study of honey collected from different flora of Pakistan. Journal of Biological Sciences, 2 (9): 626 -727.

Azeredo, L. C., Souza, S.R., Dutra, V.M, L. (2003). Protein contents and physicochemical properties in honey samples of Apis Mellifera of different floral origins. Food Chem. 80: 249 - 254.

Bhagavan, N. V. (2002). Medical biochemistry. Academic press

Ball, D.W. (2007). The chemical composition of honey. Journal of Chemical Education, 84: 643 - 646.

Beykaya, M.J. (2021). Determination of physicochemical properties of raw honey samples. Progress in nutrition, volume 23.

Bogdanov, S., 2009. Honey Composition. The honey book, pp. 1-9.

Bogdanov, S. & Martin, P. (2002). Honey authenticity, a Review. Mitt. Lebensm. Hygiene, 93: 232 - 254.

Bogdanov, S., Lullman, C., Martin, P., Russman, H., and G. Vorwohl. (2004). Honey quality. Methods of analysis and International regulatory standards: Review of the work of the international honey Commission. Swiss Bee Research Centre, EAM, Liebefeld, Switzerland.

Bogdanov, S., Baumann, E., 1988. Bestimmung von Honigzucker mit HPLC. Mitt. Geb.Lebensmittelunters. Hyg. (79) 198-206.

Buba, F., Gidado, A., & Shugaba, A. (2013). Analysis of biochemical composition of honey samples from North-East Nigeria. Biochem Anal Biochem, 2 (3), 139.

Carroll, T. and Kinsella, J. (2013). Livelihood improvement and smallholder beekeeping in Kenya: the unrealised potential. Development in Practice, 23 (3), 332-345.

Chefrour, A., Draiaia, R., Tahar, A., Kaki, Y., Bennadja, S., and Battesti, M.J. (2009). Physicochemical characteristics and pollen spectrum of some north-east Algerian honeys. African Journal of Food Agriculture Nutrition and Development, 9 (5): 1276 - 1293.

Cherian, K.J., Bhowal, M., and Godghate, S.D. (2010). Influence of floral source on chemical properties of honey. International Journal for Environmental Rehabilitation and Conservation, 1: 72-76.

Chua, L. S., Abdul-Rahaman, N. L., Sarmidi, M. R., & Aziz, R. (2012). Multi-elemental composition and physical properties of honey samples from Malaysia. Food Chemistry, 135(3), 880-887.

Codex Alimentarius Commission (2001). Recommended European regional standard for honey. (CAC/RS 12 -1969).

Crane, E. (1990). The Words beekeeping-past and present: Dadant and sons (Ed). The Hive and the Honeybee. Dadant and sons, Inc, Hamilton, Illinois, U.S.A., pp 1-38.

Eshete, Y., & Eshete, T. (2019). A review on the effect of processing temperature and time duration on commercial honey quality. Madridge Journal of Food Technology, 4 (1), 158-162.

European Union. (2002): Council Directive 2001/110/EC of 20 December 2001 relating to honey. Off. J. Europ. Comm, 10: 47- 52.

Fernandez Torres, R., Perez Bernal, J.L., Bello Lopez, M.A., Callejon-Mochon, M., Jimenez-Sanchez, J.C., Guiraum Perez, A. (2005). Mineral content and botanical origin of Spanish honeys. Talanta, 65: 686 - 691.

González-Miret, M.L., Terrab, A., Hernanz, D., Fernández-Recamales, M.Ã., Heredia, F.J. (2005). Multivariate correlation between color and mineral composition of honeys and by their botanical origin. Journal of Agricultural and Food Chemistry, 53 (7): 2574 - 2580.

Hermosín, I., Chichón, R.M. & Caezudo, M.D. (2003). Free amino acid composition and botanical origin of honey. Food Chem, 83: 263-268.

Iftikhar, F., Masood, M. A. and Waghchoure, E. S. (2011). Comparison of Apis cerena, Apis dorsata and Apis Mellifera. Honey from Different Areas of Pakistan. Asian Journal of experimental Biological Science, 2: 399 - 403.

International Honey Commission Methods (2009). Harmonized methods of the International Honey Commission Methods. Swiss Bee Research Centre, FAM, Liebefeld, Switzerland.

International Honey Commission, (2002). Honey quality, methods of analysis and international regulatory standards. Pp. 2 - 35.

Ioannidou, M. D., Zachariadis, G.A., Anthemidis, A.N., Stratis, J.A. (2005). Direct determination of toxic trace metals in honey and sugars using inductively coupled plasma atomic emission spectrometry. Talanta, 65 (1):92 - 97.

Kandagor, J., & Nyandoro, D. K. O. (2018). Analysis of livelihood diversification to food security among rural households in Ndhiwa Sub County, Homa Bay County, Kenya. Journal of Food Security, 6 (2): 90-98.

Khali,M., Moniruzzaman, M., Boukrou, L., Benhanifia, M., Islam M., Suluiman, S.A., Gan, S. H., (2012).Physicochemical and antioxidant properties of Algerian honey. Molecules, 17: (9), 11199 - 11215.

Machado De-Melo, A. A., Almeida-Muradian, L. B. D., Sancho, M. T., & Pascual-Mat, A. (2018). Composition and properties of Apis mellifera honey: A review. Journal of apicultural research, 57 (1), 5-37.

Mărgăoan, R., Topal, E., Balkanska, R., Yücel, B., Oravecz, T., Cornea-Cipcigan, M., & Vodnar, D. C. (2021). Monofloral honeys as a potential source of natural antioxidants, minerals and medicine. Antioxidants, 10 (7), 1023.

Markowicz, D.B., Monaro, E., Siguemoto, E., Sfora, M., Valdez, B. (2012). Maillard reaction products in processed foods: pros and cons. In: Valdez B, editor. Food industrial processes-methods and equipment. InTech, pp. 281- 300.

McCarthy, J. (1995). The antibacterial effects of honey: Medical fact or fiction? Am. Bee. Journal, 135: 341 - 342.

Meda, A., Charles, E. L., Marco, R., Jeanne, M. and Odile, G.N. (2005). Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey as well as radical Scavenging activity. Journal of Food Chemistry, 91: 571 - 577.

Muli, E. M., Unguti and Raina, K. (2007). Quality of honey harvested and processed using traditional methods in rural areas of Kenya, 76: 315 - 320.

Nanda, V., Sarkara, B.C., Sharmaa, H.K., and Bawab, A.S. (2003). Physicochemical Properties and Estimation of Mineral Content in honey Production from Different plants in Northern India. Journal of Food Composition and analysis, 16: 613 - 619.

Oliveira Resende Ribeiro, R., Mársico, E. T., da Silva Carneiro, C., Simoes, J. S., da Silva Ferreira, M., de Jesus, E. F. O., & Junior, C. A. C. (2015). Seasonal variation in trace and minor elements in Brazilian honey by total reflection X-ray fluorescence. Environmental monitoring and assessment, 187, 1-8.

Otieno, L. A. (2021). Determination of Physicochemical Properties of Sisal Honey from Taita Taveta County Kenya (Doctoral dissertation, Kenyatta University).

Ouchemoukh, S., Louaileche, H., and Schweizer, P. (2007). Physicochemical characteristics and pollen spectrum of some Algerian honey. Food Control, 18: 50 - 58.

Ough, C. (1991). Rapid determination of proline in grapes and wines. Journal of Food Science, 34: 228 - 230.

Persano, O. L. and Bogdanov, S. (2004). Determination of honey botanical origin: Problems and issues. Apidologie, 35: 2 - 3.

Przybylowski P, Wilczyńska, A. (2001). Honey as an environmental marker. Food Chemistry, 74: 289 - 291.

Qamar, S., Ahmed, E., Latif, F., Ali, S. S. and Shakoori, A. R. (2008). Physicochemical Analysis of Apis dorsata Honey from Tarai Forests, Nepal. Pakistan Journal of Zoology, 40: 53 - 58.

Raweh, H. S., Badjah-Hadj-Ahmed, A. Y., Iqbal, J., & Alqarni, A. S. (2022). Impact of different storage regimes on the levels of physicochemical characteristics, especially free acidity in Talh (Acacia gerrardii Benth.) honey. Molecules, 27(18), 5959.

Robert, B. M. (2010). Characterization of Kenyan Honey and a Design Model for Processing Equipment. Doctor of Philosophy in the School of Pure and Applied Sciences, Kenyatta University, Nairobi.

Salim, H.Z., Biagio, G.F., Elena, N.A., Gabriele, F.B., and Larbi, A.B. (2011). Quality Evaluation of Some Honey from the Central Region of Algeria. Journal of Biological sciences, 4: 243 - 248.

Salinas, F., Alvarez, P., Montero de Espinaza, V. and Lozano, M. (1994). Study of physicochemical parameters of honey by Rash model. Revista Espanola de Ciencia Tecnologia de Alimentos 34 (6): 672 - 683.

Seeley, T.D. (1995). The Wisdom of the Hive. Harvard University press, Cambridge.

Sousa, L.E., Marques, G., Benassi, M.T., Gullon, B., Pintado, M. M. (2016). Sugar profile, physicochemical and sensory aspects of monofloral honeys produced by different stingless bee species in Brazilian semi-arid region. Food Science and Technology, 65: 645-651.

Teshome, A., Asefa, B., & Amene, T. (2020). Physico-chemical properties of honey from different beehives and honey sources in selected Districts of Bale Zone, Oromia Regional State, Ethiopia. Journal of Bioscience and Biotechnology Discovery, 5(1), 1-8.

Teresa, G., Xesus, F., Antinio, I., and Leticia, M. (2011). Study of organic Honey from the Northwest of Portugal. pp 1430 - 3049.

Terrab, A., Diez, M.J., and Heredia. F.J. (2002). Characterization of Moroccan unifloral honeys by their physicochemical characteristics. Journal of Food Chemstry, 79: 373 - 379.

Terrab, A. Angeles, F. Recamales, D.H., Fransisco, S. and Heredia, J. (2004). Characterization of Spanish honeys by their physicochemical characteristics and mineral content. Journal of Food Chemistry, 88: 537 - 542.

Von hanen, L.P., Emmertz, A., Savage G.P. (2011). Mineral analysis of mono-floral New Zealand honey. Food Chemistry, 128 (1): 236 - 240.

WEEMA (Water, Education, Economic empowerment, Medical, and Alliance), 2016. Improving Household Livelihoods with Modern Beekeeping and Honey Production. Final Report for WEEMA International. Colombia.

White, J.W. (1975). Composition of honey, In Crane, E(ed). Honey A Comprehensive survey, Heinemann, London, pp. 157 - 206.

Yadata, D. (2014). Detection of the electrical conductivity and acidity of honey from different areas of Tepi. Food Science and Technology, 2 (5): 59 - 63.

Zappala, M.; Fallico, B.; Arena, E. and Verzera, A. (2005). Methods for the determination of HMF in honey. Journal of Food Control, 6: 273 - 277.

Downloads

Published

2023-12-13

How to Cite

Ondogo, L., Oyoo, D., & Bichanga, R. . (2023). Determination of the Chemical Properties of Honey from Suba Region, Homa Bay County-Kenya. International Journal of Food Sciences, 6(3), 1–20. https://doi.org/10.47604/ijf.2240

Issue

Section

Articles