EVALUATION OF THE ANTIFUNGAL ACTIVITY OF HYDROLYZED CAMEL WHEY PROTEIN AGAINST SOME FUNGI IN SOFT CHEESE
DOI:
https://doi.org/10.47604/ijf.1610Keywords:
Camel´s WPC, Enzymatic Hydrolysis, Antifungal Activity, Soft Cheese, Refrigerated StorageAbstract
Purpose: The current study's aim is to investigate the effect of hydrolyzed whey protein concentrate (WPC) derived from camel's milk on growth of some fungi inoculated in soft cheese during refrigerated storage.
Methodology: The pepsin-trypsin (P-T) camel´s WPC hydrolysate (20 mg/g) was incorborated in to soft cheese and their effects on the survival of Candida albicans, Asperigillus fumigatus Asperigillus flavus and Asperigillus niger (103-104cfu/g) were examined till cheese deterioration.
Findings: The results revealed that P-T hydrolysate had the ability to decrease the viability of C.albicans, A.fumigatus A.flavus and A.niger in soft cheese. C.albicans was the most sensitive strain.
Unique contribution to theory, practice and policy: Thus, camel´s WPC hydrolysates can be exploited as. This study was conducted to elaborate antifungals from camel´s WPC after enzymatic hydrolysis which could serve as a safe alternative of natural antifungal agents in soft cheese
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Abd El-Fattah, A.M. Sakr, S.S. El-Dieb, S.M., & Elkashef, H.A.S. (2017). Bioactive peptides with ACE-I and antioxidant activity produced from milk proteolysis. International Journal of Food Properties, 20(12): 3033- 3042.
Abd El Tawab, A. A., El-Hofy, F. I., EL-diasty, E. M., Abo-Hamdah, E., & AlKhayat,
M. (2020). Diversity of some food borne fungi associated with raw milk and some cheese in Egypt.Benha Veterinary Medical Journal, 38(1): 48-51.
Al-Abdalall, A.H. (2010). The inhibitory effect of camel's milk on mycotoxins and fungal growth. African Journal of Agricultural Research, 5(11):1331-1337.
Al-Majali, M.A., Ismail, Z.B., Al-Hami, Y., & Nour, A.Y. (2007). Lactoferrin concentration in milk from camels (Camelus dromedarius) with and without subclinical mastitis. International. J. appl. Res. Vet. Med,5:120-124.
Alsteens, D., Dupres, V., McEvoy, K., Wildling, L., Gruber, H.J., & Dufrene, Y.F. (2008). Structure, cell wall elasticity and polysaccharide properties of living yeast cells, as probed by AFM. Nanotechnology, 19: 38-4005.
Banjara, N., Suhr, M.J., Hallen, A., & Heather, E. (2015). Diversity of yeast and mould species from a variety of cheese types.Current Microbiology, 70(6).
Benedict, K., Chiller, T.M., & Mody, R.K. (2016). Invasive fungal infections acquired from contaminated food or nutritional supplements: a review literature of the Foodborne Pathog. Dis., 13, pp. 343-349.
Cagri, A., Ustunol, Z., & Ryser. E. T. (2002). Inhibition of three pathogens on bologna and summer sausage slices using antimicrobial edible films. J. Food Sci. 67:2317-2324.
Denis, R., Isabelle, M., & Francine, M. (1997). Selective enumeration and survival of bifeidobacteria in fresh cheese, International Dairy Journal, 7: 785-793.
El-Desoukey, R. M. A., Elbadah, B. S.A., Elqahtani, A. M. M., A Elrezq, M., and Eloseemy, R. M. (2020). Comparative Antimicrobial Study of Camel Milk and Urine against Some Animal Pathogens. EC Veterinary Science, 5(9): 134-141.
Fatchiyah, F., Hardiyanti, F., & Widodo, N. (2015). Selective inhibition on RAGE-binding AGEs required by bioactive peptide alpha-S2 case in protein from goat Ethawah breed milk: Study of biological modeling Acta Informatica Medica, 23 (2):90-96.
Garnier, F., Valence, J., & Mounier. (2017). Diversity and control of spoilage fungi in dairy products: an update Microorganisms, 5.
Guimarães, A., Ramos, O., Cerqueira, M., Venâncio, A., & Abrunhosa, L.(2020). Active Whey Protein Edible Films and Coatings Incorporating Lactobacillus buchneri for Penicillium nordicum Control in Cheese. Food and Bioprocess Technology, 13:1074-1086.
Hill, T., & Lewicki, P. (2007). Statistics Methods and Applications. Tulsa, OK. Stat Soft, USA.
Hymery, N., Vasseur, V., Coton, M., Mounier, J., Jany, J.-L., Barbier, G., & Coton, E. (2014). Filamentous fungi and mycotoxins in cheese: a review. Comprehensive Reviews in Food Science and Food Safety, 13(4): 437-456.
Jrad, Z., El Hatmi, H., Fguiri, I., Arroum, S., Assadi, M., & Khorchani, T. (2013). Antibacterial activity of Lactic acid bacteria isolated from Tunisian camel milk. Afr. J. Microbiol. Res, 7(12):1002-1008.
Konuspayeva, G., Faye, B., & Loiseau, G. (2009). The composition of camel milk: a meta-analysis of the literature data. Journal of Food Composition and Analysis, 22: 95-101.
Kumar, D., Chatli, M.K., Singh, R., Mehta, N., and Kumar,P .(2017). Antioxidant and antimicrobial activity of ultra-filtered fractions of camel milk protein hydrolysates under in-vitro condition. IndianJournalofAnimalSciences, 87 (11): 1391-1395.
Kure, C.F., Skaar, I., & Brendehaug, J. (2004). Mould contamination in production of semi-hard cheese. Int.J. Food Microbiol.,15(93):41-49.
Medeiros, B. G., dos Souza, M. P. S., Pinheiro, A. C., Bourbon, A.I., Cerqueira, M. A., & Vicente, A. A. (2014). Physical characterisation of analginate/lysozyme nano-laminate coating and its evaluation on "˜Coalho'cheese shelf life. Food and Bioprocess Technology, 7(4):1088-1098.
Miller, G.D. (2006). Handbook of Dairy Foods and Nutrition, 3rd ed. Available online:https://www.routledge.com/Handbook- Dairy-Foods-and Nutrition/Miller- Jarvis-McBean/p/book/9780367389710 (accessed on 6 January 2021).
Oxoid (2006). The Oxoid Manual, 6th Ed. Culture media, ingredients and other laboratory services. 6th Ed. Published by Unipath Limited, Wade Road, Basingstoke Hampshire, R G 24 OPW, England.
Pirkhezranian, Z.,Tahmoorespur,M., Daura,X., Monhemi,H., & Sekhavati , M.H. (2020).Interaction of camel Lactoferrin derived peptides with DNA: a molecular dynamics study. MC Genomics. Research Article, 21:60.
Ramet, J.P. (2001). The technology of making cheese from camel milk (Camelusdromedarius). Rome: Food and Agriculture Organization of the United Nations.
Seifu, E., Buys, E.M., & Donkin, E.F. (2005). Significance of the lactoperoxidase system in the dairy industry and its potential applications: a review. Trends in Food Science and Technology, 16: 137-154.
Shashikumar, C.S.S., & Puranik, D.B. (2012): Study on Use of Lactoferrin for the Biopreservation of Paneer.Tropical Agricultural Research, Vol. 23 (1): 70 - 76.
Silva, T., Adao, R., Nazmi, K., Bolscher, J. G., Funari, S. S., & Uhrikova, D.(2013b). Structural diversity and mode of action on lipid membranes of three lactoferrin candidacidal peptides. Biochim. Biophys. Acta 1828, 1329-1339.
Vogel, H.J., Schibli, D.J., Weiguo, J., Lohmeier-Vogel, E.M., Epand, R.F., & Epand, R.M. (2002). Biochem Cell Biol, 80: 49-63.
Wang, R., Han, Z., Ji, R., Xiao, Y., Si, R., Guo, F., He, J., Hai, L., Ming, L., & Yi, L. (2020). Antibacterial Activity of Trypsin-Hydrolyzed Camel and Cow Whey and Their Fractions. Journal of Animals, 10-337.
Zdybicka-Barabas, A., Stączek, S., Mak, P., Skrzypiec, K., Mendyk, E., & Cytryńska, M. (2013). "Synergistic action of Galleria mellonella apolipophorin III and lysozyme against Gram-negative bacteria. Biochim Biophys Acta, 1828 (6): 1449-1456.
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Copyright (c) 2022 Eman Abd El Samei Bakri Nafei , Prof. Dr. Ekbal Mohammed Adel Ibrahim , Prof.Dr. Hend Ahmed Elbarbary , Prof.Dr. Hamdi Abd El Samei Mohammed
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